Essential Ion Channel Methods

Author: P. Michael Conn
Publisher: Academic Press
ISBN: 9780123822376
Size: 46.40 MB
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This volume was prepared with a view toward providing a sampling of the range of molecular and physical methods that are significant for the study of ion channels.

Essential Bioimaging Methods

Author: P. Michael Conn
Publisher: Academic Press
ISBN: 9780080963426
Size: 46.94 MB
Format: PDF, ePub, Mobi
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Part of the Reliable Lab Solution series, this volume provides clear advice and explicit protocols, providing updates to classic, tried-and-true methods and an essential addition to the bookshelf or workbench of any researcher in the field. ...

Membrane Transport Proteins Advances In Research And Application 2013 Edition

Publisher: ScholarlyEditions
ISBN: 1481677527
Size: 19.81 MB
Format: PDF, Mobi
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According to the news editors, the researchers concluded: “Finally, we discuss
some single-channel studies of the anionic GlyR and GABA(A)R that prefaced
the introduction of this technique to her laboratory. ... (2012 Jun 26) University of
Valparaiso: Simple, fast and accurate implementation of the diffusion
approximation algorithm for stochastic ion channels with multiple States By a
News Reporter-Staff News Editor at Life Science Weekly — A new study on
Membrane Transport ...

Microbial Food Safety In Animal Agriculture

Author: Mary E. Torrence
Publisher: John Wiley & Sons
ISBN: 0470752602
Size: 70.50 MB
Format: PDF, Docs
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Purification of DNA from fecal, food, and environmental samples using a reliable
method is essential for achieving satisfactory results with PCR. A number of ... In
our laboratory, we have successfully used buoyant density centrifugation with
Percoll (Pharmacia Biotech) gradient medium for very sensitive detection of C.
jejuni in artificially spiked egg contents with PCR (Sabin et al. 2001a). Despite
the use ... (2001) designed an ion-channel biosensor system for detecting

American Biotechnology Laboratory

Size: 66.27 MB
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1 Membranes utilize two kinds of transport mechanism:2 Ion channels are
integral membrane proteins that form a pore to allow the passage of specific ions
by passive diffusion. Ion channels undergo conformational ... The preferred
method with the highest information content for the characterization of ion
channel function and its regulation is the patch clamp technique, which allows
reliable recording of ionic currents under a defined membrane voltage." However
, patch clamp ...

Handbook Of Toxicology Third Edition

Author: Michael J. Derelanko
Publisher: CRC Press
ISBN: 1439890137
Size: 57.79 MB
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While many assays are of potential value regarding the characterization of drug
action on the cardiovascular system, contemporary regulatory authority, and
correspondingly contemporary practice, has focused on a relatively limited set of
key indicators to be employed at a minimum prior to administration of a new
chemical entity in humans. The essential first tier tests include assessment of the
potential for cardiac ion channel interactions, and evaluation of several global
measures of ...

Scientific Essentials Of Reproductive Medicine

Author: Stephen G. Hillier
Publisher: Saunders
Size: 11.56 MB
Format: PDF
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Cultures are incubated undisturbed at 37°C with 5% CO, for 3–5 days after which
they are examined for cell growth. Harvests for chromosome analysis are usually
obtained after 4–7 days. After treatment with hypotonic solution, followed by a
series of fixations, the coverslips are placed in a stream of air to aid the spreading
of the chromosome. Chromosome analysis is routinely performed using the G-
banding method to ascertain the chromosomal complement of the fetus. In 95%
of ...

The Laboratory Computer

Author: John Dempster
Publisher: Elsevier
ISBN: 9780080521558
Size: 18.69 MB
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Equation [7.9] provides a model for noninactivating (or at least very slowly
inactivating) currents like the squid axon K channel. The complete ... The
theoretical basis of these equations lies in Hodgkin & Huxley's concepts of the
ion channel conductance being regulated by series of gates, each with individual
voltage-dependent kinetics. The Na ... A reliable non-linear curvefitting algorithm,
ideally with the capability of accepting user-defined equations, is essential for this
kind of work.

Vertebrate Viruses Advances In Research And Treatment 2011 Edition

Publisher: ScholarlyEditions
ISBN: 1464932514
Size: 41.12 MB
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According to a study from the United States, “Influenza A virus encodes an
integral membrane protein, A/M2, that forms a pHgated proton channel that is
essential for viral replication. ... SSM-based measurements of the activity and
drug sensitivity of A/M2 Wild-type and mutant channels with measurements made
with conventional electrophysiology methods, we show that SSM-based
electrophysiology is an efficient and reliable tool for functional studies of the A/M2
channel protein and ...

Wine Tasting

Author: Ronald S. Jackson
Publisher: Academic Press
ISBN: 0128018267
Size: 75.24 MB
Format: PDF, ePub, Docs
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In addition, professional panelists need to be tested for an adequate degree of
acuity, trained in the use of precise descriptive language, and be assessed for
consistent and reliable scoring. Although sensory evaluation ... Examples are
diuretics disrupting ion channels (essential for receptor cell activation),
antimicrobial agents (suppressing cytochrome P450-dependent enzymes in the
nasal mucus), and chemotherapeutic agents (affecting receptor-cell regeneration
). For consistency ...